Sentence examples for further problem of using from inspiring English sources
A further problem of using only one reference gene for normalisation, is that it theoretically should have been normalised itself prior to target gene normalisation [ 9].
The lytic antigen presentation observed by Flano et al. emphasizes further the problem of using in vitro assays as absolute measures, rather than to compare wild-type and knockout viruses (or DCs).
A further problem with using this method is the characterization of the nebulisers, although characterized in the factory before use, the nebulisers have removable components.
Even if one overcomes this problem, and could observe evidence of the discreteness of space, so many approaches involve such discreteness that one would face a further problem in using this new data to decide between the discrete approaches.
A further problem was the use of HRQL questionnaires that were in-house and/or not validated for the Spanish population.
A further problem with the use of standardized neuropsychological tests before and after the intervention to measure cognitive outcomes involves the potential for practice effects which may obscure possible effects of specific treatments.
A further problem connected with the use of the hazard index approach for the risk classification of mixtures is that a suitable reference has to be chosen.
This further amplifies the problem of footwear use at home, increasing the cumulative stress on an inadequately protected foot.
The Kremlin has acknowledged this problem, and it has cracked down on local officials accused of using their positions to further personal feuds.
