Exact(8)
Adjacent lung tissue was immersed in fixative and further prepared for histology or electron microscopy.
Tissues and swabs were subsequently processed and further prepared for DNA analysis, virus isolation, histopathology, and immunohistochemistry (see below).
Ascending aorta was removed after 8 weeks of electroacupuncture stimulation, fixed in 4% formaldehyde, and further prepared for paraffin sectioning [ 17].
The IM and RM discs were snap-frozen in liquid nitrogen, crushed to a fine powder, dissolved in elution buffer overnight, and centrifuged (13,000 × g, 10 min), and the supernatant was collected and further prepared for Western blotting [ 20].
Digital images were further prepared for presentation using Metamorph software (Molecular Devices) and Adobe Photoshop.
The NCs were further prepared for the 4 experimental conditions (Table 1).
Some time before surgery, the patient is transported to the holding department where the patient is further prepared for surgery.
Patellae were then further prepared for measurement of SO42- incorporation in the articular cartilage as previously described [ 26].
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