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Further preparation steps are required to inhibit further oxidation.
Very low or extremely deep trephination depths would, therefore, be accurately observed prior to further preparation steps, for example, leading to retrephination, or particular attention, in needle placement.
In further preparation steps bad contacts were removed, bond lengths were optimized, disulfide bonds were created, protein terminals were capped and selenomethionine residues were converted to methionine.
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Only for solid foods was a further preparation step necessary to get the terpenes (α- and β-thujone, camphor) into solution, to be used for liquid-liquid-extraction.
Further sample preparation steps and measurements were performed under less stringent safety conditions.
For plasma, no further sample preparation steps were needed, i.e. the lysates were mixed with matrix solution and deposited on a target plate.
As the critical level in alcohol products appears to be in the upper mg/L-range, the detection limit of our procedure of 0.7 mg/L appears to be adequate for the purpose, and we have refrained from further sample preparation steps (e.g., solid phase extraction) that might improve the detection limit but also increase the time and cost of the assay.
Then we present as an outlook some preliminary results obtained for further data preparation steps beyond normalization and PCA.
If drug analysis in target tissue is needed, the tissue sample has to be homogenized in a suitable medium before further sample preparation steps.
Further sample preparation steps were carried out within 1 hour after collection during which the sample was kept at room temperature.
We performed additional analyses for further data preparation steps, although with fewer datasets and fewer analysis settings than in the studies for normalization and PCA.
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