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The pelleted solids were transferred to fresh containers for freeze-drying, and the aqueous fractions were centrifuged repeatedly at 13 500 × g for 15 min intervals at 4 °C until no further pellet was observed.
The resulting pellet was saved for nuclear protein extraction and the supernatant was re-centrifuged at 2500 rpm for 10 min at 4°C until no further pellet was visible.
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A laboratory rat may pick up, chew, and swallow a pellet of food at one moment; half an hour later, after having eaten 20 grams of food, the rat will simply ignore any further pellets offered.
Further pellets were collected and supernatants were discarded.
This lysate was further pelleted by spinning at 30 000 rpm for 30 min in a Beckman L8-80M usingcentrifuge using a Type 45 Ti fixed angle rotor.
Further, the pellet was sintered at 900°C for 24 h.
Further, TWC pellet showed ∼48% improved thermal shock resistance compared to TiC, which could be due to the suppressed large thermal cracks by CNT and in-situ formed GNR.
Further, RNA pellet was air-dried for 5 10 minutes.
Dried pellets were further evaluated for pellet moisture content, unit, bulk, tapped density, and durability.
After incubation, biomass was recovered from Vacutainer tubes by centrifugation and further cell pellets were washed with N2 purged sterile distilled water twice to remove broth and LEO or citral residues.
Further, cell pellets were disrupted and dihydrofolate reductase (DHFR) activity determined under each experimental condition.
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