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After one to three further passages on rats, the vaccine was deemed safe for human use.
Adherent cells contain a majority of astrocytes whose purity was increased with trypsinization and further passages.
During further passages, rearranged segments were maintained in the reassortant viral progenies.
Prior to cell harvest, these cells were grown for three further passages on gelatine-coated plates to eliminate feeder cells.
Correspondingly, repeated air sac inoculation of a low-pathogenic H5N3 isolate in 1-day-old-chickens did not lead to high virulence for 4-to-6-week-old chickens, whereas only two further passages in brain yielded an HPAIV [49].
Late-EPCs were released from the original tissue culture plates by trypsinization (trypsin 0.25%) (Euroclone, Wetherby, UK), resuspended in EGM-2 medium, and plated onto 25 cm2 tissue culture flasks precoated with fibronectin for further passages.
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An embolus, a foreign or abnormal particle circulating in the blood, may block a vessel too small to permit further passage.
In a further passage severely damaging to the government, Haddon-Cave says: "In my view XV230 was lost because of a systemic breach of the military covenant brought about by significant failures on the part of all those involved.
In a further passage, he says "a number of the detainees described how they had, in fact, felt greatly humiliated and demeaned by having to remove all their clothes and/or by having had them forcibly removed during processing … I have heard credible evidence from a number of sources that such an order would have been particularly humiliating for an Iraqi Muslim man".
For further passaging, trypsinization was performed according to the manufacturer's instructions.
In our case, EGD confirmed the gastric contents and facilitated performance of gastric lavage, preventing further passage of drugs to the small bowel by aspiration of gastric contents.
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