Sentence examples for further passage in from inspiring English sources

Cells from the P4 Master Bank were expanded through a further passage in culture plasticware or a final passage in a perfusion bioreactor (Quantum; Terumo), to generate a P6 Working Bank.

Recombinant virus was then amplified and recovered, either after inoculation of transfection supernatant into the allantoic cavity of embryonated specific-pathogen-free eggs or after further passage in cell culture.

This suggests that abnormal cells may have been present, but were not capable of growing progressively upon further passage in vivo.

Correspondingly, repeated air sac inoculation of a low-pathogenic H5N3 isolate in 1-day-old-chickens did not lead to high virulence for 4-to-6-week-old chickens, whereas only two further passages in brain yielded an HPAIV [49].

Further passages in the presence of 800 μM R led to a reduction of diversity of population RAp45 (1.90-fold reduction in AD value, Tukey post hoc test, p < 0.001; Table 1).

In the case of ME49, a single plaque-purified clone (i.e. ME49 B7.21-E1) that was FUDRr was further passaged in a cat (cross TX332) to derive the parental clone referred to as ME49 FUDRr.

After electroporation with RNA transcripts derived from either pBeloR26_TAV or pBeloR26_E2gif a large number of CSFV NS3-positive cells could be observed (data not shown) and chimeric virus stocks, termed vR26_TAV and vR26_E2gif, were generated after further passages in cells.

To confirm that TET cells retain embryonic identity and developmental potential in vivo, cells kept in Tam for six passages were stably transfected with a GFP-expression plasmid, sorted for high GFP and after two further passages in Tam injected into mouse blastocysts.

The in vivo selected cell line was further passaged in ovariectomized athymic nude mice and re-established in vitro to generate a new cell line, MCF-7/LCC1, which is also estrogen independent but is similarly tamoxifen responsive as its parent cell line [ 30].

This virus was initially isolated in mouse brain, further passaged once in mouse brain and three times in BHK before used in our mouse experiments.

These viruses (without further passage) were diluted in Eagle minimal essential medium (MEM) containing antibiotics and 10% antibody-negative normal horse serum before being used.