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Understanding the chemical structure, physiochemical, and chemical-informatic properties of these natural product compounds will give clues for further modifications required in their structures responsible for their biological activities.
Further modifications required for formation of the known natural phenylpropenes include additional hydroxylation of the benzene ring, methylation of any of the hydroxyl groups on the benzene ring, and formation of a methylenedioxy bridge (Figure 1).
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Sortase provides a means of directly ligating expressed protein with no further modification required to solid supports.
However, further modifications are required in the ligand design to enhance the catalytic activity [99, 101].
Further modifications are required to be consistent with the DAs' manufacturing designs.
Further modifications are required to translate this system into clinical applications.
Questions were assessed to be unambiguous and socially apt; no problems were identified and hence, no further modifications were required.
Although, the ASAT been through multiple iterations and content validity testing, further modifications were required to produce the next iteration, ASAT v17.
For activation and localization of FoxO3a in the nucleus, further modifications are required, such as phosphorylation by mammalian sterile 20-like kinase 1 (MST1) and arginine methylation by protein arginine methyltransferase1.
The data extraction form was pilot tested by the team using a randomly selected set of five eligible papers until consensus was reached and no further modifications were required.
Furthermore, according to Emera and Wagner [ 205], it appears that for many genes, with examples both in the endometrial decidualization network and elsewhere [ 204, 205], the insertion of a promoter-carrying TE was not sufficient for the activation of the gene, but rather multiple further modifications were required after the insertion.
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