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We used MALDI Imaging Mass Spectrometry [ 36], whereby the different compounds present in brain sections can be detected simultaneously without further labelling.
Further labelling reactions using different combinations of serine-to-alanine mutations allowed the sites of phosphorylation to be identified, which were subsequently confirmed using single-site mutants.
The further labelling with 40 μg precursor in HEPES were performed as usual.
In our knowledge, compared with other Ag systems, Ag/AAO SERS substrate could detect a variety of small biomolecules (adenine (A), thymine (T), cytosine (C), guanine (G), β-carotene) and water pollutants (malachite green), which can be used extensively in different fields without further labelling or modification.
He asked him: "I support further labelling, but do you not think it's wrong just to look at religious slaughter in isolation from other forms of slaughter... indeed labelling on how the animals actually lived in terms of housing, food and the drug consumption that they've had.
To overcome the lack of staining for MyoD together with staining for NCAM, analysis of new sections stained for MyoD only was performed under the microscope before further labelling.
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In order to ensure that only uniformly developed samples were used for analysis, rice ears with the same heading date were labelled, and then florets on the labelled ears with the same flowering date were further labelled.
A worse injury, to his other hand, cost him two months this season and further labeled him as fragile.
This molecular worm was further labeled with Rhodamine B and then was incubated with liver cancer cells HepG2.
We further label GO DEX with a radioactive isotope, 125I, for in vivo tracking in animal studies.
Then the source points are further labeled as in-plane edges and off-plane edges by unsupervised classification technique.
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CEO of Professional Science Editing for Scientists @ prosciediting.com