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After removing the ST9 donor region, the alignment of CC398 sequences was further interrogated for recombination using the BratNextGen software (21).
Two sensitive and two resistant cell lines were further interrogated for difference in baseline and MEK162-induced gene expression, as well as signal transduction using microarray and western blotting.
The hospital radiological examination database was further interrogated for any chest radiograph or computed tomography reports containing the key words for radiological signs for pneumonia including infiltrate, consolidation, and/or pleural effusion.
Additional techniques for processing of whole mount preparations, paraffin-embedding, and microtomy were developed so that ACF and mucin-depleted foci could be further interrogated for the presence of dysplasia and for beta-catenin accumulation by immunohistochemical analysis, since beta-catenin accumulation is directly linked to colon cancer development [ 10, 23- 25].
Our 338 gene set was further interrogated for genes found in regions of copy number aberration in ovarian cancer using two datasets – The Cancer Genome Atlas and Gorringe et al. [22].
Following the screening process, candidate essential genes are further interrogated for the presence of transposon inserts using PCR and DNA sequencing.
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Fold change cut-offs and statistical analysis are the most commonly used approaches for generating gene lists which are further interrogated in terms of biological significance by gene ontology and pathway analysis [ 23].
He was held there for almost a year, at times shackled and handcuffed in a small cage with other detainees, and further interrogated, he said.
This phenomenon is of significance for the design and fabrication of protein biochips that are designed to capture bacteria from buffer or water so that these can be further interrogated with respect to possible pathogenicity.
These selections were further interrogated and sources classified as submarine, hydrothermal, fumerolic or of unknown type were discarded.
When the data were further interrogated, a reduction in the percentage osteoid surface to eroded surface quotient (OS/ES) was found for the fracture group compared with the age-matched control group suggesting a reduced adaptive modeling drift capability in the fracture group.
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