Further functional studies as well as protein and gene expression studies are needed to find out which ACh receptor types in iERMs are affected by application of different agonists and antagonists, and which one of them can further affect the proliferation and differentiation potential of the ERM cells.
The new regulatory factors and cell wall biogenesis genes that we identified provide candidate genes for further functional characterization, as well as new tools for molecular breeding and biotechnology aimed at improvement of tree growth rate, crown form, and wood quality.
Taken together, we found that SMPDL3B is a GPI-anchored glycoprotein with putative enzymatic function that efficiently co-purified with several TLRs from macrophages and was therefore chosen for further functional evaluation as potential modulator of TLR activity.
Considering this data and the previous results from the siRNA-mediated and pharmacological inhibition of FGFR4, we selected FGFR4 for further functional analysis as a novel and potentially druggable target in CRC.
In addition, however, there seem to exist further functional roles, as experimental data have demonstrated that K10 specifically inhibits proliferation and cell cycle progression of keratinocytes (Paramio et al. 1999; Koch and Roop 2004) and loss of K10 leads to increased keratinocyte turnover (Reichelt et al. 2004; for review, see Magin et al. 2007).
These processes may warrant further functional investigation, as many eukaryotic translation initiation factors were significantly modulated by BaP in lung (Eif4g2, Eif3c, Eif4g3, Eif5 Mtif2 and Eif1), in spleen (Eif5a, Eif5, Eif4g2, Eif4e and Eif1) and in forestomach (Eif3h, Eif5, Eif4e2, Mtif2 and Eif4e).
Replication of these findings is needed as well as further functional investigation of the associated alleles.
Nonetheless, the introduction of scotopic microperimetry in clinical practice is suggested to prevent further functional and organic retinal damage as much as possible.
To investigate this possibility, further functional studies such as knock-out models or other targeted inhibition experiments on these genes should be carried out.
The resulting observations are assigned to each CDS and further on contribute as basis for the determination of the gene annotation data: gene name, gene product, description, functional category, GO [ 35] numbers, etc. Apart from CDSs, further functional elements such as functional RNAs, protein domains and signal peptides are predicted and annotated.
