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The further from the array the source is, the larger the location error is.
The 12° is only for the final clicks very close to the array – for the initial clicks further from the array the angular resolution were less than 4 degrees.
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Our finding agrees with the results of a previous study using 60 mer oligonucleotides, which reported a greater effect of mismatches further away from the array surface, with the importance of a base to hybridisation efficiency described as being roughly proportional to the distance from the array surface.
Increasing the concentration of 50 kDa dextran sulphate from 15% to 17.5 or 20% did not further improve the array results.
RT-PCR analysis further validated the array data (Figure 1D).
Therefore, 17 additional patients were included in the study by placing an additional electrode further away from the sensor array on the chest.
The expression levels of 19 notable and highly regulated transcripts from the array analysis were further confirmed by real-time PCR.
Analysis of individual optical sections indicates that the linear diffuse fluorescent strands lie below the cortical array, i.e. they are further from the plasma membrane than the more stable cortical array.
For this purpose the remaining RNA from the array analyses and from further microdissected biopsies, altogether 20 CIN3 and 20 CxCa, were used.
Eight significantly down-regulated miRNAs from the array data were selected for further validation and analysis.
Repetitive regions are excluded from the Array.
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