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A secondary BMV assay guided fractionation of supernatant extract from surviving colonies led to fractions with high antiviral activity, which were then further fractionated for structural elucidation and activity determination of the compounds.
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More specifically, the initial aqueous fraction was further fractionated in aqueous and methanolic fractions.
Active RP fractions were further fractionated by normal-phase (NP) LC into eight fractions with increasing polarity.
The water soluble fraction was further fractionated into chloroform, ethyl acetate and n-butanol soluble fraction.
Ajuga integrifolia, a plant with better antimalarial effect, was further fractionated using solvent fractionation technique [ 23].
The separated fractions were further fractionated in the 2nd dimension by SDS-PAGE.
Active fractions were further fractionated and assayed.
The active fraction was further fractionated on a RPLC C18 column (Fig. 3c).
These fractions were further fractionated on Diaion HP-20 absorbent resin column (2.5 × 20 cm).
The oligosaccharides extracted from the initial hydrolysate (8.5 g) by charcoal fractionation were further fractionated by SEC chromatography.
To identify potent inhibitory fraction we further fractionated P. linteus by diverse solvents including chloroform, methanol, water and boiling water.
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