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The brains were further fixed in fresh fixative for 24 h at 4 °C.
The cerebellum was dissected out, further fixed in the same fixative using microwave irradiation (PELCO 3451 laboratory microwave system; Ted Pella), then dehydrated up to 70% ethanol and embedded in LRWhite resin (medium grade, SPI).
Dissected striatum were further fixed with the same fixative for 2 4 h at 4 °C and postfixed with 2% OsO4 in buffer and embedded in Araldite.
Tissues were harvested and further fixed with the same fixative for at least 4 h, followed by treatment with 15% sucrose in PBS for 4 h and then with 30% sucrose solution overnight.
The brain was dissected out and further fixed by immersion in Bouin fixative for 2 days.
Briefly, the fixed tissues were trimmed and further fixed for 24 hrs.
Tumors were excised from animals and further fixed by immersion in the same fixative, dehydrated and embedded in paraffin wax.
The brains were further fixed by immersion in the same fixative for 2 h at room temperature.
Embryos were removed from the uterine cavity, further fixed by immersion in the same fixative, and processed as specified in Table 1.
All animals were anaesthetized with sodium pentobarbital and perfused transcardially with freshly depolymerised 4% paraformaldehyde in 0.1 M phosphate buffer; the head was removed and further fixed for 24 h in the same fixative.
The eyecup was further fixed for 2 h and rinsed free of fixative with 0.1 M cacodylate buffer pH 7.2.
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