The sample was transferred to centrifuge bottles (250 ml) and centrifuged at 6000g for 10 min. The supernatant was collected, and the soil pellet re-extracted with further extraction buffer (50 ml), incubation at 65°C for 10 minutes and centrifuged as above.
The sample was incubated at 65°C for 1 hr, transferred to centrifuge bottles (250 ml) and centrifuged at 6000g for 10 min. The supernatant was collected, and the soil pellet re-extracted with further extraction buffer (100 ml), incubation at 65°C for 10 minutes and centrifugation as above.
Further extraction with water will extract high polarity of compounds such as phenolic acids, sugars and glycosides [ 59– 61].
The sample was then centrifuged at 4000, r.p.m. for 10 min: the cellular debris pellet was kept for further extraction, while the supernatant was extracted with 2.5 ml chloroform/isoamylalcohol (24/1) and the DNA in the upper phase was precipitated by addition of 2 volumes Solution II (1% w/v CTAB, 50 mM Tris HCl (pH 7.6), and 10 mM EDTA) and centrifugation at 8500, r.p.m. for 10 min.
Heisey [ 15, 20] found that the water extract of A. altissima leaves inhibites the growth of Lepidium satium (garden cress), and the activity of the water extract was not affected by further extraction with methylene chloride, but was greatly affected by extraction with methanol.
Nevertheless, the extracted fat-soluble compounds prevent the further extraction of the flavonoids [ 31].
The intracellular production of BioAuNPs requires further extraction and purification steps making the synthesis more complicated.
They were then frozen again in liquid nitrogen for 10 seconds, after which further extraction was performed by oscillo-vibration (25 movements per second) for 10 minutes.
The accumulated material from each sieve was removed by spatula and placed together onto cotton gauze in a Baermann funnel for further extraction followed by sugar flotation40.
He's donating £1,000 of the £7,000 he received to Greenpeace, to support "projects that aim to protest BPs further extraction of fossil fuels from the Earth".
