Some transplanted cells further differentiated into oligodendroglial progenitors, expressing NG2 (Fig. 3F), neuronal progenitors expressing Ngn2 (Fig. 3E) and astrocytes expressing GFAP (Fig. 3G).
All the cells further differentiated into the definite neural epithelial cells at days 14 17, and these neural progenitor cells were plated onto ornithine/laminin-coated coverslips at day 24 for terminal differentiation.
However, as growth factors were removed cells further differentiated into more specific neural cell types such as oligodendrocytes and astrocytes [ 14].
The most common reason for TI was travel (54.5%, n=80/147), which further differentiated into work- or family-related travel.
Isolated single cells from aborted human fetal brains were grown as neurospheres for a week and then further differentiated into postmitotic neurons in culture for 21 days in vitro (DIV) (Supplementary Figure 1A).
In addition, our ultrastructural observations provided more convincing evidence that small hepatocytes further differentiated into mature hepatocyte-like cells since they contained well-developed organelles such as mitochondria (Mi), endoplasmic reticulum (Er), lysosome (Ly) and Golgi apparatus (Go).
To quantify sputum cell subpopulations, leukocytes were differentiated from cellular debris and squamous epithelial cells and further differentiated into leukocyte subpopulations by gating strategies based on light scatter properties (forward scatter: FSc, sideward scatter: SSc) and specific surface markers.
Most recently, murine ESCs have been expanded in microplates on an automated liquid handling platform and further differentiated into cardiomyocytes by embryoid body (EB) differentiation on a separate microfluidic liquid handling workstation [22], which is optimal for high-throughput screening.
Hypoxia-exposed EBs showed enhanced differentiation into meso-endodermal lineage, and further differentiated into vascular lineage (Figs 1 and 4, and Supporting Information Fig S3).
A key step in this process is to differentiate hESCs into neural stem cells (NSCs) that can be further differentiated into neurons, astrocytes and glia.
Neural stem cells were further differentiated into astrocytes using Astrocyte Medium (ScienCell Research Laboratories) for 40 days.
