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To further delimit the putative nuclear-targeting signal, PSTVd subgenomic fragments were embedded within the intron, and recombinant reporter RNAs were inoculated onto Nicotiana benthamiana plants.
Gene expression and co-segregation analysis further delimit the region, leading to identification of a candidate gene that co-segregated with the gain-of-resistance phenotype.
To further delimit the candidate genomic region of the QTL for SPAD value, we genotyped 542 F2 plants derived from a cross between SL414 and Sasanishiki and identified 13 homozygous lines with recombination near RM3534 (Fig. 6).
Three STS markers generated in this region were amplified and hybridized to 2jz3 chromosomes, in order to further delimit the region which contains the proximal breakpoint (Figure 2 right).
Additional subcongenic strains were analyzed for skin tumor promotion susceptibility to further delimit this region.
To further delimit the binding site on coilin for WRAP53, six additional coilin fragments, spanning aa 93 291, were tested.
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It is argued that this finding supports the involvement of the right hemisphere in continuous reading and further delimits the scope of its contribution to this process.
The rice lpa1 locus was fine mapped and further delimited to a 47-kb region between markers RM3542 and RM482 (Andaya and Tai 2005).
This region was further delimited to 62 kb by fine mapping using an initial set of 1152 F3 plants generated from the F2 lines with informative recombination points.
The role of ionic channels was further delimited.
He further delimited three subgroups as the Bactris Jacq., Cocos L., and Elaeis Jacq.
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