Further, deletion of this domain blocks 80S assembly after 48S complex formation (Locker et al., 2007).
Further deletion of the C-terminus of LBP eliminated all catalytic activity (see Figure).
This lead to further deletion of items following the procedure described above, which quickly revealed all four Planning items as obsolete.
A further deletion at position −76 completely abolished luciferase expression, indicating that region −154/−76 contains the critical regulatory element for functioning of the TTV promoter.
Moreover, prediction of double deletion strategy with OptGeneKnock indicated that in addition to YALI0F21406g, further deletion of gene YALI0F15587g (d-glyceraldehyde-3-phosphate glyceronetransferase, R0487) could improve TAG production rate.
One encoded the predicted full length leptin binding domain while the other cDNAs were shorter as a consequence of different deletions in exon 9, and one had a further deletion in exon 10.
Further deletion of 10 bp between −75 to −65 caused complete loss of transcriptional activity.
For these reasons this protein specimen was used as minimal structure for further deletion studies into the large subunit.
However, further deletion of −491 bp to −242 bp abolished the PGC-1α effect on the mSIRT3 promoter (Fig. 2B).
Deletion of the 30aa motif in Nkd11 286 restored Dvl binding, whereas further deletion of the EFX motif eliminated Dvl binding (Fig. 5A).
This seemed to be caused by exposure of a transcriptional repression domain, as further deletion of −335CIP2ALuc to −108CIP2ALuc resulted again in significantly increased luciferase activity (Fig. 2D).
