Further information on chemical materials, MS and data processing parameters, tables of patient demographics and assigned metabolites, and figures of: HILIC and RP optimization and performance; HILIC and RP comparison; additional applications on adipose tissue, liver tissue and plasma samples; further data processing; and pathways analysis.
Further data processing, including normalization and data analysis was performed and is described in detail in the Result section.
Flex control v. 3.3 software was used for the acquisition of spectra, and all further data processing was carried out using Flex analysis v. 3.3 software.
The sequence cleaning step is an essential first step of the sequence processing pipeline before any further data processing in order to allow accurate downstream analysis.
Further data processing, including sequence extractions, multiple sequence alignments, and statistical analysis, were accomplished with Geneious Pro software (version 5.5.3, Auckland, NZ) with occasional minor manual adjustments [ 41].
The data was taken from Agilent MassHunter software after analysis and exported into Microsoft Excel for further data processing.
With the increasing applications oFurtherolomics technology on both microbial andatainical samprocessingpatincludingh appropriate conormalizationpect to witnescalingoss expandion ofilteringwerege on microbial metabolites, including the discovery of novel metabolites and performed biomarkers for diagnosis of infections such as tuberculosis.
Dependent variables were dropped from further data processing, when they were not adding discriminative power to the cluster analysis (i.e. they were equally expressed in both clusters).
We were able to schedule irrigation just from the visual analysis of the curves derived from the ZIM outputs, without any further data processing.
After closing of the database for this analysis (31 December 2001), all data were transferred to the IMI for further data processing.
