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Burns goes further: "Cultures are blending now.
Pulsatile chonic treatment was carried out by incubating cultured cells with BMP7 for 15 h followed by replacing with fresh medium and further cultures of two days.
Further cultures which have been implemented in the model are canola (oilseed rape), cup plant (S. perfoliatum), and the short rotation coppices poplar and willow, although no validation for these cultures has been performed so far.
The wound was closed in a standard fashion, but employing as few sutures as possible so as not to leave an excessive amount of foreign material in a potentially infected surgical site, and two suction drains (whose tips were sent to the microbiology laboratory for further cultures) were placed.
Therefore, all further cultures were performed in 75 µl instead of 200 µl medium.
Further cultures were again negative.
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Cells were washed and cultured in with fresh medium without OVA for further culture overnight.
"Thank you, you have done your part to further culture in the North Country, " he continued, addressing the boat's owner and leaving a Post-it he considered sufficient and self-explanatory.
No further culture supply was given to the seeds.
The GF filter paper layer was used as a sample for further culturing.
After transfection and further culturing, the cells were fixed, stained with DAB, dehydrated, and then embedded in resin as previously reported (Fig. 1B) (Martell et al. 2012).
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