Cells were washed and cultured in with fresh medium without OVA for further culture overnight.
"Thank you, you have done your part to further culture in the North Country, " he continued, addressing the boat's owner and leaving a Post-it he considered sufficient and self-explanatory.
No further culture supply was given to the seeds.
These three-dimensional structures are patterned into prostate organoids by further culture in Matrigel for 20 30 days, with prostate-specific medium.
(2) CEO were primed by forskolin and FSH for different times and then transferred into an HX-medium for a further culture.
In particular, under our experimental conditions, the immersion media was diluted, and the final concentration was 25% (v/v) to avoid further culture medium dilution and thus adverse effects on cell physiology.
Cells were washed and resuspended in DMEM for further culture.
Further culture and expansion were conducted according to the manufacturer's protocol.
The positive fraction was used for in vivo experiments without further culture.
For example, human RPE cultures models would be hard to obtain from CHM patients, and further culture and manipulate.
Without further culture medium adaption, VP-SFM and ExCell media showed high fluctuations and lower cell growth rates.
