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No further conversion was observed (Fig. S 1).
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Further conversions were performed to calculate F n fluxes in g/ha/day as follows (Eq. 2): F_{text{n}} {text{g/ha/day}} = {text{N}}_{2} {text{O}};{text{g/ha}} cdot 24, h cdot frac{A}{10000}.
It is found that by increasing temperature, conversion increases, but after 450 °C no further increase in conversion was observed, rather the conversion dropped down with further increase in temperature.
Moreover, no further 4-TBP conversion was exhibited in filtrate after removal of the catalyst.
In order to investigate further whether this conversion was appropriate for free living data we used an existing data set of children, aged 12 to 13 years (mean age 13.1 years) and adolescents aged 16 to 17 years (mean age 17.1 years) who concurrently wore both an combined movement and heart rate sensor and a 7164 Actigraph during free living (n = 50).
Even with further irradiation higher conversion was not achieved.
The reaction with 100 mg mL−1 cells gave a maximum conversion of 69 % after 17 h and, even after a prolonged reaction time (4 days), no further increase in conversion was observed.
We suggest that regressing FDCs synthesise fewer proteins within the ER, including PrPc, and thus no further PrPc/PrPd conversion is possible.
However, no further evidence for gene conversion was reported.
This conversion was further confirmed by Sanger sequencing of positive cells.
However, no further increase in percentage conversion was observed at 250 rpm, which may be due to shearing of the enzyme molecules.
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