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For further controlling the specificity of the assays, signals were analyzed in HCT-116 parental and Bax-KO cells.
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To further control the specificity of induced phenotypes in Eph4ΔC expressing cells, two other mutant forms of Eph, Eph4TM and Eph3ΔC, were used.
In addition, we performed a set of controls to further validate the specificity of the PLA technology.
Primary antibody omission controls were used to further confirm the specificity of the immunohistochemical labeling.
Furthermore, normal mouse IgG was used as a control for the HPA mAbs to further prove the specificity of the nanoparticle.
To further determine the specificity of the antibody, adsorption controls were done using SERT peptide, which resulted in total absence of target labelling.
Primary antibody omission controls and cPLA2 knockout (KO) spinal cord sections were used to further confirm the specificity of the immunofluorescence double labeling, and secondary antibody omission controls were used to determine the degree of autofluorescence.
We further checked the specificity of the labeling by using as controls ascitic fluids against yellow fever, West Nile or dengue type-1 viruses[10] [10].
This case showed maintained FISH signals, further demonstrating the specificity of the assay.
We discuss further below the specificity of the results in the Spanish context.
To further confirm the specificity of the aptamer for immunohistochemistry, we performed peptide blocking.
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