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There were no significant changes (P > 0.05) when the hydrolysis time was further continued from 10 h to 24 h.
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After adding 200 μl of Buffer AP2 (Qiagen DNeasy Plant Minikit) the mix was placed on ice for 5 min. Further steps continued from step 4 of the Qiagen DNeasy Plant Minikit protocol without modifications [ 26].
However, OD measures remained stable during the first 2 weeks (p = 0.93), dropped significantly between week 2 and week 8 (p = 0.005), and although further decline continued from week 8 to week 24, this did not reach statistical significance (p = 0.36).
Buffer AP2 (200 μl, Qiagen DNeasy Plant Minikit) was added and this was incubated on ice for 5 min. Further steps continued from step 10 of the Qiagen DNeasy Plant Minikit as described by the manufacturer's protocol (Qiagen: DNeasy plant handbook 07/2006) without modifications.
In June 2002, the network was expanded further, and routes were continued from Tampere onwards to Oulu and from Jyväskylä to Kuopio.
Continued from Figure 3.
In the second stage, these first vessels connect and further expand by angiogenesis that continues from day 32 after conception until delivery [ 11].
Further steps continued without deviation from step 4 of the Qiagen DNeasy Plant Minikit protocol (Qiagen, DNeasy Plant Mini and DNeasy Plant Maxi Handbook 01/2004).
Many students continue from 106A to further develop their skills in CS 106B.
Monitoring for further reactions continues, and from these data a drug's uses, contraindications, and limitations can be outlined.
All patients who consented to further contact continued to receive treatment from their general practitioner in the form of general advice about the condition and prescription for an analgesic where appropriate (for example, paracetamol or co-codamol).
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