Following the separation of each read into individual FASTA files representing each barcode, further contaminant control and adapter and primer removal was performed.
The cleanup would begin only after extensive testing to further pinpoint contaminants, and the owners would pay for the testing as well as the cleanup.
Further, soil contaminants show a large degree of spatial variation which increases the burden on sampling costs.
The security vacuum across the country leaves sites open to looting, which could further disperse contaminants.
For contamination residing in the vadose-zone, mixing a reactive agent in solid form is often difficult to manipulate and using a liquid reductant runs the risk of further spreading the contaminants to previously uncontaminated zones.
The advantages of phytoremediation are a high remediation level that is not compromised to the physical and chemical methods, low cost, environmental safety, the possibility of further extraction of contaminants from the green mass of plants [12, 13], and monitoring the cleaning process.
A later study using a newer advanced model demonstrated complete removal or a further reduction in contaminants by these filters.
RNA extraction was performed according the Trizol manufacturers' protocol, followed by a sodium acetate precipitation to further remove any contaminants.
