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Following the separation of each read into individual FASTA files representing each barcode, further contaminant control and adapter and primer removal was performed.
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The fragments and powders were stored in inert plastic containers, properly plugged (to avoid further contaminants) and tagged with a number indicating the sampling zone: specific details of these zones are given where appropriate in the results section.
Despite the cold, dark Arctic winter, there was considerable pressure to complete the clean-up operation before the sea ice melted in the spring and deposited further contaminants into the sea.
However, a lower second elution step could also result in co-eluting further contaminants.
Sorption of contaminants in nanopores of plastics may further inhibit contaminant biodegradation [62].
The cleanup would begin only after extensive testing to further pinpoint contaminants, and the owners would pay for the testing as well as the cleanup.
Further, soil contaminants show a large degree of spatial variation which increases the burden on sampling costs.
The security vacuum across the country leaves sites open to looting, which could further disperse contaminants.
The agency's plan, which is subject to public hearings, would provide money for two wardens to be on duty around the clock, increased public outreach and education and further monitoring of contaminant levels in commercially sold fish.
These cells were further depleted of contaminant T and B cells using magnetic beads coated with rat monoclonal antibodies to mouse CD3 or B220 (Dynal Biotech, Paisley, United Kingdom).
There is therefore scope for further refinements of contaminant exposure risk models to increase their predictive power and reduce their reliance on precautionary assumptions which tend to inflate the estimated risks.
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CEO of Professional Science Editing for Scientists @ prosciediting.com