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The recovered ds cDNA was further concentrated down to 3 μl by a speed vacuum device.
For analysis, aqueous samples were liquid/liquid extracted in dichloromethane and the collected extract was dried using anhydrous Na2SO4 (granular, 10 60 mesh, Fisher) and further concentrated down to 1 mL under a gentle N2 stream.
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The NA-containing fractions were pooled and concentrated down for further analysis.
The reaction mixture was stirred at room temperature for 2 hr and then concentrated down to dryness to obtain 100 mg of (1r,3r -cyclobutane-1,3-bis aminium 1r,3r -cyclobutane-1,3-bis aminium 1r,3r -cyclobutane-1,3-bis aminium 1r,3r -cyclobutane-1,3-bis aminium
The reaction mixture was stirred at room temperature for 1.5 hr and then concentrated down to dryness to obtain 100 mg of (1 s,3 s -cyclobutane-1,3-bis aminium) ditrifluoroacetate which was -cyclobutane-1,3-bis aminiumation.
The protein-depleted serum was concentrated using 4-ml 5 kDa molecular weight cutoff Vivaspin centrifugal concentrators (Sartorius, Goettingen, Germany) at 3200 g and 10 °C, the buffer was exchanged with distilled water and further concentrated before drying down in a SpeedVac (Thermo Scientific, Waltham, MA, USA).
The gold was concentrated by washing away the lighter river sands with water, leaving behind the dense gold particles, which could then be further concentrated by melting.
We further concentrated DGAT1 with ultra-filtration (Fig. 2A).
These moves further concentrated economic gains at the top, while leaving out most of the rest of America.
Cytosolic fractions were further concentrated with acetone precipitation.
miRNAs were further concentrated with the flashPAGE Fractionator System (Ambion).
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