By contrast, Mn in Rubus foliage was up to 100 times more concentrated than in most plant species, and was further concentrated by ∼60% during senescence.
If the final protein concentration was below 1 mg/ml, the preparation was further concentrated using Vivaspin-6 or -20 (5000 MW PES, Sartorius AG, Göttingen, Germany).
The gold was concentrated by washing away the lighter river sands with water, leaving behind the dense gold particles, which could then be further concentrated by melting.
We further concentrated DGAT1 with ultra-filtration (Fig. 2A).
This solution was further concentrated to 2 mL by a stream of nitrogen.
The ethyl acetate extracts were further concentrated using a rotary evaporator.
This solution was further concentrated to 2 mL with a stream of high purity nitrogen.
The eluent was further concentrated in a rotary vacuum evaporator (Buchi, Switzerland) before being transferred to a glass vial.
The lowest celestial sphere, the lunar sphere, also generated luminosity, which moved inwards and further concentrated the matter below this sphere.
It contains ~17 to 21% casein, with most serum proteins and lactose removed by microfiltration and diafiltration, and it is then further concentrated using vacuum evaporation.
The modified products were further concentrated under vacuum to 50 mL by using a rotary evaporator (EYELA, Tokyo, Japan) at 47 °C.
