This research was further completed with a detailed analysis of bright light (city) growth covering 160 different countries.
After the collection, the information that allowed the detection and identification of an obstruction case was further completed with various direct or indirect survey techniques.
It is noteworthy to mention that we have implemented a broad, though not exhaustive, panel of kernels, which is susceptible to be further completed with other base kernels or kernel combinations (composite kernels) [48, 75, 76].
For recombinant individuals, genotyping was further completed with 13 additional SNP markers (Additional file 1).
The GI examination was then further completed with oesophageal manometry, gastric emptying scintigraphy, antroduodenojejunal manometry, colonic transit time and full-thickness biopsies of the distal ileum, depending on the clinical picture [ 5, 6, 9, 10, 12- 14].
To prevent incoming victims from realising their fate, Treblinka II was disguised as a transit camp for deportations further east, complete with made-up train schedules, a fake train-station clock with hands painted on it, names of destinations, a fake ticket window, and the stop sign "Ober Majdan", a code word for Treblinka commonly used to deceive passengers departing from Western Europe.
Given the lack of detailed biochemical data available for C. beijerinckii, we integrated and cross-checked information from three major annotation databases to reconstruct the core metabolic network, and then further completed the network with computational algorithms and manual curation.
The initial list of 1100 indicators needed to be further completed in order to comply with the evolving legislation, with the specific demands of the Belgian authorities and also to reduce it to a number of practically implementable indicators.
The one-step labeling of IMP467 was optimized further, completing the process within 30 min with only one SPE-purification step and a specific activity of 115 GBq/μmol (52% yield) [25].
We further completed the forward and reverse sequencing with new primers specific to each PtaLIM gene (Pta stands for P. tremula × P. alba; Supplementary Table S1).
