Further characterization indicated that the OsGRAS23 protein was mainly localized in the cell nucleus, and that it showed obvious trans-activation activity and DNA binding ability in yeast cells (Fig. 6 and Fig. 8).
Further characterization indicated that this inhibition (i) is sensitive to the dose of TFIID used in the reaction, (ii) can be alleviated by the addition of more TFIID after chemical treatment, but not by the addition of any other protein factors, confirming that TFIID is the most likely target of inhibition in the reaction, and (iii) is insensitive to various mutations in core promoter elements.
Moreover, with CH4 as the carbon source, vertically-aligned CNT forests of ∼12 μm in height are achieved, and further characterizations indicate that the forests consist of dominant triple-walled nanotubes with an average inner tube diameter of 3.2 3.7 nm mostly.
Further characterization indicated no differences in their proliferation rate (data not shown).
Although in SEC and other biophysical assays the impression could arise that these structures are homogenous, further extensive biophysical characterization indicates that the size distribution of these oligomeric aggregates is rather heterogeneous in nature, ranging from 80 to 500 kDa, although their morphology is protofibrillar.
Further biochemical characterization indicated that both PitA-1 and PitA-2 have weaker Fe3+-binding ability than their protein complex.
The lack of reversion to a wild-type phenotype along with further basic molecular characterization indicated that these characteristics are encoded on a single large plasmid which has been lost in the non-pigmented variants [ 20, 23].
Further investigation on flocculation mechanism via pH monitoring, zeta potential measurements, floc properties analyses and spectral characterization indicated that, pairwise interactions among CND, copper II) and TC were present in bridging flocculation, including charge attraction, coordination and hydrophobic effect.
