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To investigate this further, cell proliferation was monitored using an EdU (5-ethynyl-2′-deoxyuridine) incorporation assay.
The cells in each microwell gradually aggregated and then spontaneously formed a single EB within 1 d of culture, and EB size increased with further cell proliferation.
Further, cell proliferation investigated by MTS assay suggests this was achieved without sacrificing biocompatibility, with no significant difference to either empty-well or commercially-pure Ti controls noted.
Further cell proliferation leads to the expansion of the whole cluster.
Cellular senescence is a fundamental cellular program that is activated after a finite number of cell divisions and operates to avoid further cell proliferation.
Ralji cells were next incubated in the presence of HDACi for 24 hours followed by γ-irradiation to prevent further cell proliferation and co-culture with purified T lymphocytes from healthy donors.
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Furthermore, aligned microchannels were produced within porous hydrogels to further promote cell proliferation.
We suggest a modification of the tissue engineering paradigm, where resident cells are attracted by endogenous, dentin-derived growth factors that further induce cell proliferation and differentiation and a bioactive scaffold material laden with these growth factors that serves as a template for tissue formation.
Treatment with PD98059 and heparinase further reduced cell proliferation and decreased cell attachment to ECM in 3-D.
After 5-days of YC137+ICI treatment, YC137 further decreased cell proliferation after treatment with ICI (Figure S2B; ANOVA p<0.001).
Further, our cell proliferation data also indicates a higher proliferation rate in virgin ALDH positive MECs.
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