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To further generate endothelial-specific CD146 knockout mice (CD146EC-KO mice), CD146floxed/floxed mice were further bred to Tek+/Cre mice (Strain Name: B6.Cg-Tg(Tek-cre)12Flv/J, The Jackson Laboratory), which specifically expressed Cre recombinase in ECs.
As Tek+/CreCD146floxed/floxe mice (CD146EC-KO mice) were viable, these mice were further bred to Tek+/+CD146floxed/floxed mice (WT mice), resulting in 50%% CD146EC-KO mice and 50 % WT mice, both of which were used for subsequent investigations (Fig. 1B).
NOD mice (NOD/ShiLtJ) and C57BL/6J mice were purchased from Jackson Labs, Bar Harbor, ME, and either used for experiments or were further bred in house.
Due to the redundancy of Smad1 and Smad5 expression and function, these mice were further bred with Smad5+/− heterozygous mice to incrementally decrease overall BMP/Smad-signaling.
Positive founders overexpressing truncated LMα1 chain in the neuromuscular system (lines No. 3 and 4) were further bred with dy3K/+ mice [2], followed by sib breeding to generate LMα2 chain deficient animals that express δE3LMα1 chain (dy3K/δE3 mice).
A total of 82 F2 hybrids were further bred from these F1 hybrids.
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A lot of his mates thought he had gone mad, but since he founded the Rare Breeds Survival Trust in 1973, no further breeds have been lost".
Natural variation was used along the history by farmers to domesticate and further breed for crop plants with desirable traits [1], [2].
The key question addressed in this study was whether migratory PGCs could be isolated and cultured from a further breed of chickens and form functional gametes and viable offspring.
Confining refugees to island camps not only immiserated them further, but bred a new lucrative market for smugglers.
Mutant ybx1 sa42 zebrafish (which harbor a V83F amino acid substitution) were propagated further and bred to homozygosity.
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