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Differences found in mRNA decay according to CD154 genotypes suggest that this polymorphism may contribute to the regulation of mRNA expression, although further assays will be necessary to elucidate its role in disease predisposition.

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Further, other assays will be required to identify and quantify the modified lipids and proteins [9], [45], [46] that are widely believed to accompany ARM progression.

More precise characterization of stem cells with identification of new, unique markers; improvement in isolation and transplant techniques; and further development of functional assays will ultimately lead to new therapies for a host of human lung diseases.

Future development of specific methods to assess the risk of bleeding in thrombocytopenic patients using global clotting assays will further improve patient management.

Further protein-RNA binding assays will elucidate whether Hfq directly interacts with these sRNAs or if the stabilization of the sRNAs by Hfq is indirect.

Carefully designed epidemiologic studies that evaluate NoV prevalence in children with diarrhea and a suitable comparison group and that use sensitive molecular assays will help further define target groups that would benefit from vaccines and other interventions.

Advances in genetic research (specifically in gene therapy; development of new, genetically engineered mouse models; and study of gene expression using DNA array assays) will contribute to further understanding the functioning of the urinary bladder in health and disease.

Further studies using antigen-specific ELISPOT assays will determine the frequency and specificity of these T-cell responses.

There is further evidence that classical T cell assays will have to be used to measure responses in the most appropriate tissue compartment [ 17, 18].

Further comparative genomic studies and functional assays will be necessary to determine if Kr-h1's function truly diverges between social and nonsocial Hymenoptera, and between different insect orders, or if the sequence differences are simply due to the accumulation of mutations during evolution in different lineages.

Further testing of candidate elements in transgenic assays will be needed to validate this possibility.

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