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30.5% of sequences were further annotated using annotation transfer procedure for Gene Ontology (GO) terms for molecular function, biological process and cellular component.
Unigene with NR annotation was further annotated and classified under Gene Ontology (GO).
The mapped GO terms were initially annotated with the default annotation threshold (55) and un-annotated sequences were further annotated at a threshold of 45.
These sequences were further annotated using the BLAST2GO annotation pipeline.
Based on BLAST and pfam annotations, melon unigenes were further annotated with Gene Ontology (GO) terms.
Variant data were further annotated by Annovar to provide functional annotation of variants.
The differentially expressed genes were further annotated for their biological themes using DAVID functional annotation tools (http://david.abcc.ncifcrf.gov/) [ 44].
These experimentally-supported predicted proteins were further annotated by assigning the proteins with standardized nomenclature and functional annotation.
Transitions are shown on the branches and transversions are further annotated by adding suffixes.
After selection of candidate somatic variants by PHIAL (as described above), the variants were further annotated by a curation team.
The 64 unique missense variants were further annotated by alignment with the BRCA1 tBRCT aa sequence (Supplementary Table S9).
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