This assumption was further evidenced in post-hoc sensitivity analyses, namely reclassification analyses, using different biomarker specific cut-off ranges and estimates from the above-mentioned models (see Additional file 2).
Interpolated climatic variables for the study area (temperature, precipitation, annual radiation) were retrieved from the meteorological model Aurelhy (Météo-France, [46] downscaled at a 100 m resolution. For further analyses, we retained the two uncorrelated variables, namely Elevation (El) and Annual Radiation (Arad).
> -wrap-foot> Subsequently, we selected CpG sites that were present >10 times in the mapping, namely CpG10, and subjected them to further analyses.
For further analyses, we retained only CNVs applying a more stringent criterion, namely CNV regions (CNVRs) containing overlapping CNVs recalled by at least two programs, spanning three or more consecutive SNPs and detected in a minimum of two animals.
To detect the expression of the four groups of CSRGAs in IL5211S, one CSRGA representative of each group, namely, CSRGA17, CSRGA22, CSRGA23, and CSRGA25, was used in further analyses.
Therefore, we decided to use only the calls at the ends of the continuum, namely 'cackles' and 'whines' and to discard the 'arcs' from further analyses.
Five hybridoma clones (namely BH1, BH2, BH5, BH6 and BH7) were selected for further analyses, based on their reactivity with HER2 ECD, but not EGFR ECD (Additional file 1).
Individual clones of stable double transfectants, namely MiaPaCa-2-TREx-TP53 and DanG-TREx-TP53, were selected for further analyses (see Invitrogen User Manual).
Among the six tree crops, three species, namely poplar, grapevine and castor bean, were found to have large-scale expression datasets for further analyses.
See Supplementary Figure 2 for further analyses.
