The different, virtual argHX sequences were further aligned with their homologues argH(A) and an evolutionary tree was reconstructed using a maximum likelihood approach (Fig 5).
The empirical data were further aligned with distinct environmental settings to associate possible factors contributing to UHIs.
Polymorphic loci and/or regions of interest in the genomes were extracted and further aligned with ClustalX [ 32].
These sequences can be further aligned with each other to identify specific shared divergent regions, which can become new primers for upcoming analyses.
Some creators, like Martell, are going a step further, aligning with corporate partners who might serve as a buffer between event creators and the bureaucratic red tape, confusing logistics, and discriminatory practices of venues or city government.
All coding sequences having one or more blast hits (other than themselves) with an e-value smaller than 10-30 (9,166 for C. elegans) were retained, translated and were further aligned together with an end-gap-penalty free global alignment [ 28], using the BLOSUM62 matrix.
Contigs with a viral hit were further aligned against the nt database and analyzed as in the two previous steps.
All reads aligned to the viral genome where further aligned against the nt database with blastn and analyzed as in the previous steps.
The pair further aligned themselves with the Egyptian deities Isis and Osiris, also married siblings.
"That is, it had no policy on Afghanistan on its own, and whatever Pakistan said, we bought". The United States was reluctant to criticize Pakistan as it further aligned itself with the Taliban after Kabul's fall.
The mid-century debate over the figurative and the abstract — which Greenberg's coining of the term "post-painterly abstraction" did much to further — aligned the figurative with illusion: the illusion of depth in a canvas, and the pretense of three-dimensional human life on what was, in truth, an inert, two-dimensional surface.
