All alignments were further aligned to resequencing data from P. taeda (unpublished data) using the profile-profile option in MUSCLE[ 59, 60].
Next, the reads at each CAL are further aligned using gapped local alignment to identify the best match.
The 3D structures of the compounds were further aligned on Hoechst 33342 using flexible and pharmacophore alignments.
The images were then further aligned and classified by several rounds of multireference alignment (sxmref_ali2d), where only high quality classes were used as references, followed by k-means classification.
All coding sequences having one or more blast hits (other than themselves) with an e-value smaller than 10-30 (9,166 for C. elegans) were retained, translated and were further aligned together with an end-gap-penalty free global alignment [ 28], using the BLOSUM62 matrix.
The aligner was then retracted and pressing with tweezers on two sides further aligned the microchips.
These sequences were further aligned through http://moss.nibb.ac.jp/cgi-bin/selectNalign.
The pair further aligned themselves with the Egyptian deities Isis and Osiris, also married siblings.
The empirical data were further aligned with distinct environmental settings to associate possible factors contributing to UHIs.
Uber, which has viewed the Middle East as an important area in its expansion, said the investment further aligned the company with Saudi Arabia as the kingdom planned to transform its economy, reducing its dependence on oil and improving employment.
"That is, it had no policy on Afghanistan on its own, and whatever Pakistan said, we bought". The United States was reluctant to criticize Pakistan as it further aligned itself with the Taliban after Kabul's fall.
