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The mycelia were harvested by washing with cold distilled water (200 ml) over a sintered glass funnel using a vacuum.
Add 25 ml of 0.1 N hydrochloric acid and stir occasionally over a period of 30 min. Vacuum filter the slurry through a medium porosity fritted-glass crucible or small funnel, using a fine stream of water from a wash bottle to aid quantitative transfer of the sample.
The resulting yellow precipitate was filtered on a medium frit sintered glass funnel using a house vacuum, immediately washed three times with ice-cold anhydrous isopropanol (3 × 35 mL), followed by ice-cold anhydrous ether (3 × 50 mL), and air-dried using house vacuum for 7 h.
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If you don't have a funnel, use a colander to strain the vodka into a bowl.
Create a model funnel using paper or soft cardboard that you can use as a mold.
The acidified water phase was thus defatted in a separatory funnel using n-hexane (3 × 25 mL).
It allows anyone to build a sales funnel by either using a templated version, or by creating your own.
We will adjust for funnel plot asymmetry using a method by Rücker et al. [ 31, 32].
Using a funnel, add celery seeds and peppercorns to bottle.
Strain juice through a fine sieve into a pitcher (or using a funnel, into a clean, empty wine bottle).
4 Using a funnel, transfer the sharbat into bottles while it is still hot.
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