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To observe cells that expressed the tagged fluorescent protein, cells were adhered to a glass funnel filter and fixed by immersion in 100% methanol at −80°C.
Funnel Filter.
Insert the funnel filter.
If the water starts to fill the funnel filter area, you will need to remove some water.
Once the water is at the appropriate level and the funnel filter has been inserted, fill the funnel with your coffee grounds.
Note that in most macchinettas, the Top Chamber, Central Column and Filter Plate are combined in one piece, the Funnel Filter is a separate piece, and the Steam Release Valve and Bottom Chamber are combined in one piece.
Similar(54)
The homogenate was vacuum filtered using a funnel with filter paper.
(Above right, the funnel and filter stored in a water glass).
"[Parents] are such a major funnel or filter of the broader stressful experiences in kids' lives," Lengua said.
The mixture was filtrated through a fritted funnel, the filter cake was washed with 10 mL THF and the filtrate was concentrated under reduced pressure.
The sample was moved into a round bottom flask through a funnel and filter paper containing 25 g sodium sulphate anhydrous and evaporated to dryness (Rotavapor Laborota 4000, Heidolph, Schwaback, Germany).
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