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Cells were collected from each culture using a sterile filter paper/Buchner funnel assembly in a sterile hood and the cell pad was washed with sterile water.
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Biomass samples harvested in the course of experiments were separated from the media with the use of vacuum filtration assembly (G4 glass frit funnel).
When sink drain design excluded the direct application of an in-line air filter, the sampling assembly was modified to include an inverted funnel.
The funnel served as the preferred respirator holder since the entire FFR/funnel assembly could be placed in a rack to dry without handling the FFR.
Overall, a unique 2-dimensional carpet-like assembly composed of large coexisiting ribbon-like, tubular and funnel-like structures with a clearly resolved protofilament substructure is observed.
However, a significant number of parasites attached to the macrophage surface and triggered the assembly process of filopodium-like, lamelopodium-like and even funnel-like structures.
After the assembly, six columns were placed on a wooden structure with funnels to collect the leachate into containers.
Place the threaded glass capillary tubes in to the vial, guide the small end of the 1D casting funnel over the tops of the tubes and press the snap cap vial onto the assembly.
The barrier that exists between the cytoplasmic and periplasmic funnels at low pH is removed, and the two funnels are bridged by hydrogen bonds between water molecules and residues located in the TMSs IV/XI assembly and helix X at alkaline pH.
When Sheldon Silver, New York's former assembly speaker, was indicted, local outlets noted that Sedita received a $3,500 contribution from the company Silver was accused of funnelling cash to.
Hence the funnel.
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