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The MIC values for bacterial strain were determined using the serial microplate dilution method developed by Eloff [ 24] while the MIC for fungi was determined using the microplate serial dilution modified by Masoko et al. [ 25].
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Protein concentration was determined using a BCA Assay Kit Piercee).
Proteasome activity was determined using fluorescence assays.
Survival was determined using Kaplan Meier test.
Peptide binding was determined using ELISA.
Iodine uptake was determined using radioactive iodine.
Signal intensity was determined using GeneChip Operating Software 1.4.
Seven parameters temperature, pH, percentage of medium volume per flask volume (% v/v) and glucose, KNO3, K2HPO4 and soy isolate concentrations affected the biomass and ARA production of this fungus and were determined using the Plackett-Burman statistical design technique.
Relative abundances of 6 well-studied bacterial taxa, total anaerobic fungi, ciliate protozoa, methanogenic Archaea and bacteria were determined using validated primer sets by real-time quantitative PCR.
Gene families were determined using OrthoMCL approach comparing with other 13 fungi (Additional file 2: Table S1).
Minimum inhibitory concentrations (MICs) were determined using a modified version of the CLSI microdilution protocol for filamentous fungi (M38-A2) and yeasts (M27-A2) [ 17, 18].
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