This sampling regime (different quadrants, several neighboring gardens per quadrant, three fungal samples per garden from different garden parts) aimed at maximizing the chance of finding genotype differences between cultivars within the large Atta nests.
Fungal sample was collected, purified and sequenced.
Lane 2: 28S rDNA amplicon of fungal sample.
The same method was used for all the fungal samples.
Inclusion criteria: Every patient admitted to ICU with positive fungal samples.
CryoSEM offers high resolution for frozen fungal samples, whereas environmental SEM allows the analysis of robust samples (e.g. spores) under ambient conditions.
The fungal samples were curated by a team led by Kasthuri Venkateswaran, a senior research scientist at NASA's Jet Propulsion Laboratory, who goes by Venkat for short.
We obtained fungal samples from 62 soil cubes.
Nest information and sample sizes are summarized as follows: Nest 2: N09.1522° W79.7361°, 22 m×14 moundnd area, 75 fungal samples from 25 gardens.
For seven nests, fungal samples from at least two gardens were genotyped (average of 3.7 gardens genotyped per nest, range 2 8 gardens) (Supporting Information Table S2).
