Here we investigate the regulation of spindle shape during spindle formation in a quantitative manner.
GSK3 has been implicated in a diverse range of cellular functions including the regulation of mitotic spindle dynamics and chromosomal alignment [2], [3], [4], [5].
CDK5RAP2 is also essential in the regulation of the spindle checkpoint; its loss causes chromosomal missegregation and decreased spindle checkpoint protein expression through binding with their promoters and transcriptional regulation in HeLa cells [ 37].
In this study, we have continued investigation into the role of CtBPs in breast cancer cell survival, identifying a previously unknown function for CtBPs in the regulation of the mitotic spindle checkpoint.
Extensive studies have identified and characterized more than a dozen genes that play important roles in the regulation of the spindle checkpoint in mammalian cells.
Survivin has been implicated in the regulation of the mitotic spindle checkpoint, from kinetic core to spindle assembly; it's over expression in cancer may allow cells with spindle defects or misaligned kinetic cores to continue through cell division.
Moreover, reduced Aurora A function correlated with aberrant centrosomal recruitment of the D-TACC/MSPS complex, which plays a role in the regulation of astral microtubules of the spindle.
Localization and function of CDC-42 have been mainly studied in one- and two-cell embryos, where CDC-42 participates in the regulation of cell polarity and spindle orientation.
Here we will review mechanisms mediating genomic instability in MM cells with an emphasis placed on pathogenic mutations affecting DNA recombination, replication and repair, telomere function and mitotic regulation of spindle attachment, centrosome function, and chromosomal segregation.
Given the recent explosion of studies indicating a role for noncoding RNA in the regulation of various cellular processes, it will be interesting to determine what types of RNAs are associated with the mitotic spindle, and whether these RNAs play an active or passive role in spindle morphogenesis.
