Because MYC and CYCLIN-D1 play important roles in regulating cell cycle progression and proliferation46,47, the effect of DAXX inhibition on these cellular functions was analysed in PTEN-deficient cells.
The diaphragmatic function was analysed by ultrasonography, performed at day 1 (reference), then days 1 and 7, postoperatively.
HBMVEC migratory function was analysed using a Boyden chamber assay.
A wide range of biomarkers and their relation to lung function was analysed simultaneously.
MiR-29a function was analysed by invasion assays and the orthotopic transplantation mouse model.
Platelet function was analysed in whole blood by flow cytometry according to the methods described by Riba et al. [ 19].
Animals from each of seven different ages (0, 6, 12, 24, 36, 48 and 60 days after birth) were obtained and their kidney function was analysed.
The same pattern of associations was observed when cognitive function was analysed as a continuous variable across fourths of cotinine concentration for both basic models (P for trend <0.001) and fully adjusted models (P for trend 0.025).
One week post-rotenone (or DMSO) administration mice were given 40 μg of MnCl2 intravitreally (20 mg/ml in PBS) into the treated eye and optic nerve function was analysed by measuring MEMRI signal 2 h later.
