These data suggest that mutS, mutL, and mutS2 cooperatively function in the repair of oxidative DNA damage (Fig. 1B) and that the loss of this function leads to the induction of biosynthesis of vitamin B1 as an ROS scavenger.
Since it has also been reported that the main driving force of the stationary phase-induced mutagenesis may be oxidative stress [19], it is expected that MutS and MutL can function in the repair of oxidative DNA damage during the stationary phase.
Other notable enzymes detected include a chloride peroxidase (Krad0128), catalyzes the decomposition of hydrogen peroxide, and methionine sulfoxide reductase (Krad 1091, MsrA), functions in the repair of oxidized proteins; peptides for each were detected from the 0.75 and 1.5 mM Cu II) grown cultures at 22 hr and 32 hr (Table S3).
An interesting member of this cluster is RAD1, which functions in the repair of double strand breaks that lack overlapping end sequences [ 80].
Several genes for homologous recombination and nonhomologous end joining, which function in the repair of DNA double-stranded breaks, were also upregulated (Additional file 1: Figure S9).
As the epsilon subunit of DNA polymerase III contains 3' → 5' exonuclease activity and is involved in DNA mismatch repair, this DnaQ-like protein may also function in the repair of DNA damage [ 60].
In animal models of Alzheimer's disease, amyotrophic lateral sclerosis (ALS), and multiple sclerosis, microglia possibly function in the maintenance, repair or regeneration of the microenvironment [21], [54], [55].
A genetic analysis of S. cerevisiae slx1 and slx4 deletion phenotypes identifies these genes to function in the recombination-mediated repair of stalled replication forks (Fricke and Brill 2003; Deng et al. 2005).
We believe that they can become wound healing fibroblasts and perform an important function in the repair of skin dermis after injury.
