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We chose arp1 and jnm1 as query genes, because an SGA-screen will detect genes functioning in pathways that compensate for the loss of dynein-dependent spindle positioning and thus also play a role in spindle migration.
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These changes are not a consequence of a single protein but rather involve multiple proteins that function in pathways and networks.
In this study, we tested whether septins function in pathways controlled by G1 cyclins and identified a septin-dependent pathway that controls the pattern of cell growth early in the cell cycle.
The finding that Shs1 is essential for viability and for septin localization in cells that lack Cln1 and Cln2 suggested that it may function in pathways regulated by G1 cyclins.
For instance, genes often function in pathways, and proteins often cluster in evolutionary families.
They function in pathways whose down-regulation leads to life span extension.
Wild-type p53 directly activates a number of genes that function in pathways of DNA repair, including a ribonucleotide reductase gene, p53R2, involved in repair of DNA damage.
These data suggest that mDia1 may induce filopodium protrusions either by binding IRSp53 or independently, whereas mDia2 always functions in pathways independent from IRSp53.
However, a number of key STE family members function in pathways distinct from MAP kinase pathways in the yeasts, for example Cdc15p/Cdc7 (discussed above), which forms part of the MEN/SIN late mitotic network.
Those abnormal expressions of those pituitary hormones significantly functioned in pathway systems such as MAPK-signaling system described above, and were involved in pathophysiological processes.
In a conventional genetic analysis, genes that function in parallel pathways with the same functional output generally demonstrate additive interactions.
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CEO of Professional Science Editing for Scientists @ prosciediting.com