TAR cloning requires that the cloned DNA fragment carry at least one autonomously replicating sequence (ARS) that can function as the origin of replication in yeast.
These common features of S. minutum minicircle CNS suggest that it functions as the origin of replication (Zhang et al. 2002).
TAR cloning requires that the cloned DNA fragment carry at least one autonomously replicating sequence (ARS) that can function as the origin of replication in yeast, which prevents wide application of the method.
Thus, if a repulsive mechanism is functioning, as initially suggested by the phenotype, the origin of the signal remains to be determined.
There is a great variability in efficiency for the methods tested as a function of the origin of the HTs (Figure 2A).
However, tissue regeneration-based assays indicate that only a subset of basal cells can function as the cell-of-origin for PCa6, whereas studies in genetic mouse models show that PCa can originate from both basal and luminal cell lineages and that luminal cells are even more susceptible to tumourigenesis9,13.
Other properties of the PBC function, as well as the origin of its name, are explained in the discussion section.
The base skews have been found closely related to genome function domains, such as the origin of replication, gene distribution, transcription and replication direction in bacteria [ 3- 5], plants [ 6] and mammals [ 7- 9].
WorldFengur is the database that contains and functions as the studbook of origin of the Icelandic horse.
However, if the causation is true, this would mean that the bidirectional promoters can function as an origin of natural dsRNAs that are specifically cleaved.
The first conserved region, immediately downstream of the coding regions, consists of approx. 150 bp and includes a large hair-pin loop which may function as an origin of replication.
