The first one is green fluorescent protein (GFP), which functions as a reporter of the global folding state of the fusion protein.
We have developed a transgenic mouse that functions as a reporter of ER activity, termed ER action indicator (ERIN), by incorporating a transgene with an estrogen-responsive promoter (three copies of the vitellogenin estrogen response element with a minimal thymidine kinase promoter) linked to the reporter gene beta-galactosidase.
For a lux-based system to function as a reporter in whole animal BLI, the resulting signal must be detectable using commercially available equipment designed for this purpose and be easily distinguishable from background light emissions.
Analyses of arrays where a catalyst was printed at variable spot concentrations confirm that the IR complex of the circuit can function as a reliable reporter of the levels of immobilized catalyst.
We recently demonstrated that IFP also functions as an excellent reporter for protein expression in the protozoan Leishmania tarentolae [21].
Recently, we demonstrated that IFP also functions as an excellent reporter for protein expression in Leishmania tarentolae [21], a unicellular eukaryotic protozoan for recombinant protein production [22].
This region can function as a promoter in reporter assays and includes a TATA box and a transcription initiation site of DUX4 [ 12, 16].
We corroborated the qPCR result using the dap k07309 enhancer trap line, which functions as a dap-LacZ reporter (Mitchell et al., 2010).
At least one sequenced copy of D4Z4 contains a single-exon open reading frame (ORF) and an upstream region that can function as a promoter in reporter assays [ 12].
Feilmeier, B.J., Iseminger, G., Schroeder, D., Webber, H. & Phillips, G.J. Green fluorescent protein functions as a reporter for protein localization in Escherichia coli.
