The technology breakthrough of RNA interference (RNAi) and with it the ability to functionally screen the whole genome, made it feasible to use functional genomics to dissect components of the Smad nuclear import pathway.
Assays for five different hydrolase enzymatic assays were conducted with five substrates to functionally screen the fosmid library.
We then employed the rodent malaria laboratory model of P. berghei [6] [12] to functionally screen candidate genes in the PRI.
The top-ranked 37 compounds were further functionally screened for cytotoxicity by using NB cell lines.
Finally, optimized sequences would be synthesized, inserted, and functionally screened in the relevant host.
In this paper we have functionally screened six candidate genes from the 1p36.2 region (CORT, DFFA, ENO1, ICAT, PEX14 and PGD) in neuroblastoma cells.
Finally, Addis et al. employed a calcium reporter to functionally screen candidate factors and define a five factor cocktail of Hand2, Nkx2.5, Gata4, Mef2c, and Tbx5 (HNGMT) for maximally efficient reprogramming of functional iCMs (Addis et al., 2013).
Genes identified through screening and functionally related.
Given that heterologous GPCRs can be expressed functionally in nociceptive neurons, we sought to investigate the structure-function relationship by screening SST-14 analogues.
Here, we exploited the ground-state culture system to screen functionally for genes promoting exit from naive pluripotency.
