For example, electrophysiology and functional imaging do not visualize connectivity, and tracers are not targeted to functionally activated neurons.
We investigated the interaction of TRPV4 with cytoskeletal components biochemically, cell biologically by observing morphological changes of DRG-neurons and DRG-neuron-derived F-11 cells, as well as functionally with calcium imaging.
The default mode network consists of a set of functionally connected brain regions (posterior cingulate, medial prefrontal cortex and bilateral parietal cortex) maximally active in functional imaging studies under "no task" conditions.
Targeting of "functionally integrated" PLNs to tail clots was confirmed via fluorescent imaging.
HCM-associated JPH2 mutations were engineered and functionally characterized using immunocytochemistry, cell morphometry measurements, and live cell confocal calcium imaging.
In summary, by imaging neural activity at cellular resolution during naturalistic behavior, we have functionally identified circuit components that are likely to mediate the perceptual recognition of ethologically relevant stimuli and the release of an adaptive behavioral response.
Functionally, two separate optical paths are required: one for fundus illumination and another for imaging.
Several of the brain regions found to be the most central and functionally connected are areas that have previously been reported as active during language tasks in imaging studies.
By combining automated imaging with siRNA screening of these cells, we identified 43 signal-transducing receptors and secreted factors with functionally validated roles in mediating the in vitro growth of primary normal human mammary epithelial cells.
Modern imaging techniques are now enabling us to unravel the various ways in which nature uses such organizational principles to functionally diversify the subcellular landscape.
