We and others have shown that PPARγ is expressed and functionally active in chondrocytes and that PPARγ activators modulate the expression of several genes considered essential in the pathogenesis of OA.
Both the longer and shorter isoforms contain the major functional domains of the protein, including the GTPase domain, and are functionally active in biochemical assays [ 29, 36].
As for the G-to-C transition at position –173 of the MIF gene, this site became functionally active in the presence of C through the creation of an activator protein 4 transcription factor binding site.
Relatively low cell adhesion was observed on uncoated collagen nanofibers, whereas collagen nanofibrous matrices treated with type I collagen or laminin were functionally active in responses in normal human keratinocytes.
The purified proteins were functionally active in flow cytometry, immunofluorescent microscopy, and competition binding experiments performed to delineate the epitopes recognized by different monoclonal antibodies against the same polypeptide.
It was found that nanofibrous membranes made of PLGA/collagen were functionally active in responses in human fibroblasts, and were very effective as wound-healing accelerators in early-stage wound healing.
These results were consistent with the idea that OsNCED4 is functionally active in ABA biosynthesis.
The HEK293F-derived, recombinant hENT1 is homogenous and functionally active in proteoliposome-based counter flow assays.
Like OsNCED3, OsNCED4 is functionally active in ABA biosynthesis in rice.
In this study, reverse genetic approaches were used to determine if rice OsNCED4 is functionally active in ABA biosynthesis.
These results indicated that in addition to OsNCED3, OsNCED4 is also functionally active in ABA biosynthesis at least in Arabidopsis.
